Mouse anti-CD71 (H68.4) antibody reacts with the N-terminal region of the human transferrin receptor. Due to the conserved amino acid sequences of the cytoplasmic tail among species, this antibody is able to cross-react with chicken, mouse and Chinese hamster CD71. Cross-reactivity has also been observed with rat adrenal pheochromocytoma (PC-12).
The human transferrin receptor (TR; CD71) is a homodimeric type II transmembrane glycoprotein consisting of two identical 95 kDa subunits covalently linked by intermolecular disulfide bonds in erythroid blood cell line and in activated leukocytes. CD71 has an extracellular domain of 671 amino acids, a single 28-residue transmembrane region, and a 61-residue N-terminal cytoplasmic domain. Expression of the receptor is limited on normal tissue, however, highly expressed on the surface of some tumors. CD71 is included in the class of ligand transport receptors which are internalized by clathrin-mediated endocytosis. An important function of the receptor is to bind the serum transport protein transferrin (TF; apotransferrin) and mediate the uptake of iron into the cell. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron. A growing area of research involves the use of monoclonal anti-CD71 antibodies to inhibit the uptake of iron and thus block tumor cell growth.
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Protein Aliases: CD71; mammary tumor virus receptor 1; p90; sTfR; T9; TFR; TFR1; TR; Transferrin receptor protein 1; Transferrin receptor protein 1, serum form; TRFR
Gene Aliases: 2610028K12Rik; AI195355; AI426448; AU015758; CD71; E430033M20Rik; I79_000642; IMD46; Mtvr-1; Mtvr1; p90; T9; TFR; TFR1; TFRC; TR; TRFR
Molecular Function: receptor