Immunohistochemistry analysis of CD71 showing positive staining in the cytoplasm and membrane of paraffin-treated Human placenta tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CD71 monoclonal antibody (Product # MA5-11441) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Hamster, Sheep, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:2|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:40|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 3 publications below|
MA5-11441 reacts with human and mouse samples in IHC (P), FACS and Western blot applications. This antibody is not suitable for MCF-7 or NIH-3T3 cells in Western blot analysis.
Cell surface transferrin receptor (CD71) is a disulfide-bonded homodimeric glycoprotein. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. Transferrin receptor (CD71) has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Artemisinin derivatives induce iron-dependent cell death (ferroptosis) in tumor cells.
MA5-11441 was used in immunocytochemistry to study the induction of iron-dependent cell death, ferroptosis, in tumor cells by artemisinin derivatives
|Ooko E,Saeed ME,Kadioglu O,Sarvi S,Colak M,Elmasaoudi K,Janah R,Greten HJ,Efferth T||Phytomedicine : international journal of phytotherapy and phytopharmacology (22:1045)||2015|
Spatial and temporal organization of tick-borne encephalitis flavivirus replicated RNA in living cells.
MA5-11441 was used in immunocytochemistry to study the spatial and temporal organization of tick-borne encephalitis flavivirus replicated RNA in living cells
|Miorin L,Maiuri P,Hoenninger VM,Mandl CW,Marcello A||Virology (379:64)||2008|
Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus.
MA5-11441 was used in immunocytochemistry to characterize the putative epidermal stem cells from Cashmere goat fetus
|Islam MS,Zhou H||European journal of dermatology : EJD (17:302)||2007|