|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Transferrin receptor 1.|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Western Blot (WB)||1:50|
|Western Blot (WB)||See 1 publications below|
MA1-40198 detects Transferrin receptor 1 from human samples.
MA1-40198 has been successfully used in flow cytometry, ELISA, immunohistochemistry (frozen), immunoprecipitation, and Western blot applications.
The MA1-40198 immunogen is human Transferrin receptor 1.
Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.
Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells.
MA1-40198 was used in western blot to elucidate the role of transferrin receptor and intracellular iron in hepcidin secretion.
|Mehta K,Greenwell P,Renshaw D,Busbridge M,Garcia M,Farnaud S,Patel VB||Blood cells, molecules & diseases (55:110)||2015|
CD71; TFR; TFR1; transferrin receptor protein 1; TRFR
CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR