Immunofluorescent analysis of Transferrin Receptor / CD71 in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a Transferrin Receptor / CD71 polyclonal antibody (Product # PA5-27739). Green: Primary antibody at a dilution of 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 169 and 407 of Human CD71|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27739 targets CD71 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-27739 immunogen is recombinant fragment corresponding to a region within amino acids 169 and 407 of Human CD71.
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.