|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human TGase II|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/mL|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||5-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16272 targets Transglutaminase II in IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16272 immunogen is recombinant full length human TGase II.
TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Exercise, vascular stiffness, and tissue transglutaminase.
PA5-16272 was used in western blot to study the effects of aerobic exercise on NO signaling, transglutaminase-2 activity and vascular stiffness in a rat model
|Steppan J,Sikka G,Jandu S,Barodka V,Halushka MK,Flavahan NA,Belkin AM,Nyhan D,Butlin M,Avolio A,Berkowitz DE,Santhanam L||Journal of the American Heart Association (3:null)||2014|
A novel extracellular role for tissue transglutaminase in matrix-bound VEGF-mediated angiogenesis.
PA5-16272 was used in western blot to study the role of transglutaminase-2 in an angiogenic mechanism mediated by ECM-bound VEGF-A
|Wang Z,Perez M,Caja S,Melino G,Johnson TS,Lindfors K,Griffin M||Cell death and disease (4:null)||2013|
Increased tissue transglutaminase activity contributes to central vascular stiffness in eNOS knockout mice.
PA5-16272 was used in western blot to study the increased vascular stiffness of mice ablated for eNOS and the role of elevated levels of transglutaminase-2
|Jung SM,Jandu S,Steppan J,Belkin A,An SS,Pak A,Choi EY,Nyhan D,Butlin M,Viegas K,Avolio A,Berkowitz DE,Santhanam L||American journal of physiology. Heart and circulatory physiology (305:H803)||2013|
Transglutaminase 2 interaction with small heat shock proteins mediate cell survival upon excitotoxic stress.
PA5-16272 was used in immunoprecipitation and western blot to study the role of transglutaminase-2 and small heat shock proteins in mediating cell survival upon excitotoxic stress
|Caccamo D,Condello S,Ferlazzo N,Currò M,Griffin M,Ientile R||Amino acids (44:151)||2013|
Transglutaminase 2 is secreted from smooth muscle cells by transamidation-dependent microparticle formation.
PA5-16272 was used in immunocytochemistry to study the role of transamidation-dependent microparticle formation in the mechanism of transamidase-2 secretion by smooth muscle cells
|van den Akker J,van Weert A,Afink G,Bakker EN,van der Pol E,Böing AN,Nieuwland R,VanBavel E||Amino acids (42:961)||2012|
The redox state of transglutaminase 2 controls arterial remodeling.
PA5-16272 was used in immunocytochemistry to study regulation of type 2 transglutaminase
|van den Akker J,VanBavel E,van Geel R,Matlung HL,Guvenc Tuna B,Janssen GM,van Veelen PA,Boelens WC,De Mey JG,Bakker EN||PloS one (6:null)||2011|