Immunofluorescence analysis of Transthyretin was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Transthyretin (10E1) Mouse Monoclonal Antibody (LFMA0174) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Cytoskeleton was stained with alpha-Tubulin Monoclonal Antibody (Product# PA516891, 1ug/ml) followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 594 conjugate (A11037) (Panel c: red). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human protein purified from E.coli|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control for this product is human Plasma.
This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Autoimmune response to transthyretin in juvenile idiopathic arthritis.
LF-MA0174 was used in ELISA and western blot to characterize transthyretin and its autoimmune response in juvenile idiopathic arthritis
|Clement CC,Moncrieffe H,Lele A,Janow G,Becerra A,Bauli F,Saad FA,Perino G,Montagna C,Cobelli N,Hardin J,Stern LJ,Ilowite N,Porcelli SA,Santambrogio L||JCI insight (1:null)||2016|