|Tested species reactivity||Fruit fly, Human, Mouse, Non-human primate, Rat, Xenopus, Yeast, Zebrafish|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is tri-methylated|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of mixed lineage leukemia 1(MLL1) protein as a coactivator of heat shock factor 1(HSF1) protein in response to heat shock protein 90 (HSP90) inhibition.
MA5-11199 was used in ChIP assay to study how the cellular changes induced by HSP90 inhibition affect cancer.
|Chen Y,Chen J,Yu J,Yang G,Temple E,Harbinski F,Gao H,Wilson C,Pagliarini R,Zhou W||The Journal of biological chemistry (289:18914)||2014|