HeLa cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS. The fixation step stabilizes the morphology of the cells and permeabilizes membranes. (A) Cells were labeled with the H3K4me3 Polyclonal Antibody, Rabbit (diluted 1:200 and incubated for 1 hour at room temperature) followed by a FITC-labeled goat anti-rabbit secondary antibody. (B) Nuclei were stained using the DNA-specific stain DAPI. Both, the H3K4me3 Polyclonal Antibody, Rabbit and DAPI produced clear nuclear staining.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against the region of the histone H3 containing the trimethylated lysine 4 (or [K4me3]), using a KLH-conjugated synthetic peptide.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1 ug|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post tranlationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Epigenetic drift towards histone modifications regulates CAV1 gene expression in colon cancer.
49-1005 was used in ChIP assay to analyze epigenetic drift towards histone modifications and how they regulate CAV1 gene expression in colon cancer
|Deb M,Sengupta D,Kar S,Rath SK,Roy S,Das G,Patra SK||Gene (581:75)||2016|
|Not Applicable||Not Cited||
Cross-species genomic and epigenomic landscape of retinoblastoma.
49-1005 was used in ChIP assay to analyze the cross-species genomic and epigenomic pattern of retinoblastoma
|Benavente CA,McEvoy JD,Finkelstein D,Wei L,Kang G,Wang YD,Neale G,Ragsdale S,Valentine V,Bahrami A,Temirov J,Pounds S,Zhang J,Dyer MA||Oncotarget (4:844)||2013|
Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.
49-1005 was used in immunocytochemistry to study why HSC function declines with age
|Flach J,Bakker ST,Mohrin M,Conroy PC,Pietras EM,Reynaud D,Alvarez S,Diolaiti ME,Ugarte F,Forsberg EC,Le Beau MM,Stohr BA,Méndez J,Morrison CG,Passegué E||Nature (512:198)||2014|