Western blot analysis of Tri-Methyl Histone H3 (Lys9) in Hela histones using a Tri-Methyl Histone H3 (Lys9) monoclonal antibody (MA5-18065) at various dilutions. Samples were separated by SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was blocked in 3% NFDM-PBST for 1 hr at room temperature and incubated at 4°C prior to analysis.
|Tested species reactivity||Bovine, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide of Human Histone H3, tri methylated at K9|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-dependent|
|Dot blot (DB)||Assay-dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for K9 tri-methylated histone H3 and shows weak cross-reactivity with K9 di- and monomethylated histone H3; does not react with unmethylated K9. Predicted to react with many other species due to sequence homology, such as mouse, rat, rabbit, hamster, pig, S. cerevisiae, Drosophila and non-human primates.
This target has a predicted molecular weight of 15 kDa. Actual Western blot results show a band at 17 kDa. This can be blocked using tri-methyl K9 Histone H3 peptide.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.