Immunofluorescent analysis of Tri-Methyl-Histone H3 (Lys9) in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a Tri-Methyl-Histone H3 (Lys9) polyclonal antibody (Product # PA5-31910). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 6 and 20 of Human Histone H3|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5 µg|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31910 targets Tri-Methyl-Histone H3 mLys9 in WB applications and shows reactivity with Human samples.
The PA5-31910 immunogen is synthetic peptide corresponding to a region within amino acids 6 and 20 of Human Histone H3.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
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