Western blot analysis of Tri-Methyl Histone H4 (Lys20) in Hela cell lysates using a Tri-Methyl Histone H4 (Lys20) monoclonal antibody (Product # MA5-18074) at various dilutions, followed by detection using an AP-conjugated mouse IgG whole molecule antibody at a dilution of 1:2000 on a BCIP/NBT substrate.
|Tested species reactivity||C. elegans, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide of Human Histone H4|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-dependent|
|Dot blot (DB)||Assay-dependent|
|Western Blot (WB)||1:4000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody shows weak cross-reactivity with K20 di-methylated histone H4, but does not react with histone H3 or mono- or unmethylated K20 histone 4. This antibody is predicted to react with most mammals based on sequence homology.
This target has a predicted molecular weight of 11 kDa.
Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.