Invitrogen

Triadin Monoclonal Antibody (IIG12)

Tech Support

Datasheet

Protocols

Tech Support

Product Image Gallery

Triadin Antibody in Western Blot (WB) — Published figure using Triadin monoclonal antibody (Product # MA3-931) in Western Blot

Product Details

MA3-931

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	1:500	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse, Rabbit
Published species	Mouse, Rabbit
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	IIG12
Immunogen	Rabbit skeletal muscle triads.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2256496

Product Specific Information

MA3-931 detects triadin from mouse and rabbit skeletal muscle tissues. This antibody does not detect triadin from cardiac tissues.

MA3-931 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a single ~95 kDa band representing triadin in rabbit skeletal muscle extracts. Immunofluorescence staining of triadin in rabbit skeletal muscle with MA3-931 results in its co-localization with ryanodine receptor, which is consistent with the association of ryanodine receptor and triadin in triads.

The MA3-931 antigen is rabbit skeletal muscle triads.

Target Information

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR's) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR's), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR's and RyR's. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

S-glutathionylation decreases Mg2+ inhibition and S-nitrosylation enhances Ca2+ activation of RyR1 channels.

The Journal of biological chemistry

Aracena P,Sánchez G,Donoso P,Hamilton SL,Hidalgo C

MA3-931 was used in western blot to investigate the effect of S-glutathionylation and S-nitrosylation on the modulation of RyR1 channels by Mg2+ and calcium.

Fri Oct 31 00:00:00 EST 2003

S-glutathionylation decreases Mg2+ inhibition and S-nitrosylation enhances Ca2+ activation of RyR1 channels.

The Journal of biological chemistry

Aracena P,Sánchez G,Donoso P,Hamilton SL,Hidalgo C

MA3-931 was used in western blot to investigate the effect of S-glutathionylation and S-nitrosylation on the modulation of RyR1 channels by Mg2+ and calcium.

Fri Oct 31 00:00:00 EST 2003

Supramolecular calsequestrin complex.

European journal of biochemistry

Glover L,Quinn S,Ryan M,Pette D,Ohlendieck K

MA3-931 was used in western blot to investigate the formation of supramolecular calsequestrin complex

Sun Sep 01 00:00:00 EDT 2002

Calsequestrin binds to monomeric and complexed forms of key calcium-handling proteins in native sarcoplasmic reticulum membranes from rabbit skeletal muscle.

Biochimica et biophysica acta

Glover L,Culligan K,Cala S,Mulvey C,Ohlendieck K

MA3-931 was used in western blot to study the binding of calcium handling proteins to calsequestrin in rabbit skeletal muscle sarcoplasmic reticulum membranes

Sat Dec 01 00:00:00 EST 2001

Low-frequency stimulation of fast muscle affects the abundance of Ca(2+)-ATPase but not its oligomeric status.

Journal of applied physiology (Bethesda, Md. : 1985)

Harmon S,Froemming GR,Leisner E,Pette D,Ohlendieck K

MA3-931 was used in western blot to investigate the possible effects of stimulation-induced changes in the abundance of calcium pump on protein-protein interactions

Mon Jan 01 00:00:00 EST 2001

Identification of a novel 45 kDa protein (JP-45) from rabbit sarcoplasmic-reticulum junctional-face membrane.

The Biochemical journal

Zorzato F,Anderson AA,Ohlendieck K,Froemming G,Guerrini R,Treves S

MA3-931 was used in western blot to characterize a novel 45kDa protein isolated from rabbit skeletal muscle sarcoplasmic reticulum junctional face membranes

Sun Oct 15 00:00:00 EDT 2000

Effects of chronic low-frequency stimulation on Ca2+-regulatory membrane proteins in rabbit fast muscle.

Pflugers Archiv : European journal of physiology

Ohlendieck K,Frömming GR,Murray BE,Maguire PB,Leisner E,Traub I,Pette D

MA3-931 was used in western blot to study the effects of chronic low frequency stimulation of fast-twitch muscle on the levels of Ca(2+) regulatory membrane proteins

Fri Oct 01 00:00:00 EDT 1999

A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function.

The Journal of cell biology

Moore RA,Nguyen H,Galceran J,Pessah IN,Allen PD

MA3-931 was used in western blot to characterize a transgenic myogenic cell line without functioning skeletal muscle ryanodine receptor.

Mon Feb 23 00:00:00 EST 1998

Characterization and ultrastructural localization of a novel 90-kDa protein unique to skeletal muscle junctional sarcoplasmic reticulum.

The Journal of biological chemistry

Guo W,Jorgensen AO,Campbell KP

MA3-931 was used in immunohistochemistry to study a novel 90 kDa protein specifically localized to the junctional SR of rabbit skeletal muscle

Fri Nov 11 00:00:00 EST 1994

Phosphorylation of the 1,4-dihydropyridine receptor of the voltage-dependent Ca2+ channel by an intrinsic protein kinase in isolated triads from rabbit skeletal muscle.

The Journal of biological chemistry

Imagawa T,Leung AT,Campbell KP

MA3-931 was used in immunoprecipitation to characterize the intrinsic protein kinase in triads isolated from rabbit skeletal muscle and its function

Mon Jun 15 00:00:00 EDT 1987

Bioinformatics

Protein Aliases: cardiac triadin; junctional sarcoplasmic reticulum glycoprotein; Triadin

Gene Aliases: TRDN

Triadin Monoclonal Antibody (IIG12)

Product Details

MA3-931

Western Blot (WB)

Immunohistochemistry (IHC)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!