|Tested species reactivity||Chicken, Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length native protein (purified) (Chicken).|
|Storage buffer||PBS, pH 7.4, with 0.6% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21228 detects Tropomyosin in Human, Rat and Chicken samples.
MA1-21228 has been successfully used in Immunofluorescence and Immunohistochemistry (Paraffin) procedures. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, p.H. 6.0, for 10 minutes followed by cooling at room temp for 20 minutes.
MA1-21228 immunogen corresponds to full length native protein (purified) (Chicken).
Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.