Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Troponin and tropomyosin are the main proteins responsible for calcium regulation of actin-myosin interactions. Tropomyosin is a rod-shaped molecule that binds in the groove of the actin helix, and prevents the attachment of the myosin fiber heads. The troponin complex consists of three polypeptide subunits, troponin T, troponin I, and troponin C. Troponin T (for tropomyosin binding unit) attaches the troponin complex to tropomyosin which results in tropomyosin being situated in the myosin-binding site on the actin molecule. Troponin I (for inhibitory subunit) binds to actin and troponin T, and this complex inhibits actin-myosin binding even in the presence of calcium. Troponin C (for calcium-binding subunit) binds to troponin T and troponin I, and makes the complex dependent upon calcium. With calcium present, troponin C binds up to 4 molecules and changes the position of the troponin and tropomyosin complex, so that myosin is able to bind to the actin filament. Troponin I is present in three different isoforms in mammals, a slow-twitch skeletal muscle-specific form, a fast-twitch muscle-specific form, and a cardiac muscle-specific form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cardiac muscle; TNNC1; troponin I; troponin I type 1 (skeletal, slow); troponin I, skeletal, slow 1; Troponin I, slow isoform; Troponin I, slow skeletal muscle; Troponin I, slow-twitch isoform
Gene Aliases: 2700018B22Rik; AI747285; SSTNI; TNN1; TNNI1