Immunohistochemical analysis of Troponin T/TNNT3 in paraffin-embedded human skeletal muscle following steamed antigen retrieval with a citrate buffer, pH 6, using a Troponin T/TNNT3 polyclonal antibody (Product# PA5-37899) at a dilution of 5ug/ml. Detection was performed using an AP-conjugated secondary antibody.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-TTLRSRIDQAQKHSK, from the C-terminus of TNNT3|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||4-8 µg/ml|
|Western Blot (WB)||0.01-0.03 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.