Western blot analysis of Human Brain Tissue Lysate using PA5-19749, Tryptophan Hydroxylase primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19749 staining of Mouse Brain Tissue Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19749 staining of Serotoninergic nucleus Rat Tissue Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Tryptophan Hydroxylase.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 42 kDa.
This antibody is predicted to react with rabbit, chicken, hamster, chimpanzee, gorilla and orangutan based on sequence homology.
This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.