Western blot analysis of TSC2 was performed by loading 20ug of two independent 293T cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in PBST for 1 hour at room temperature. The membrane was probed with a TSC2 monoclonal antibody (Product # MA5-15004) at a dilution of 1:1000 at 4°C overnight, washed in PBST, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:2000 for 1 hour at room temperature. As a loading control, the same lysates were probed with an antibody against alpha-Tubulin. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide from the amino terminus of human tuberin|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/ml BSA|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
It is not recommended to aliquot this antibody.
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.
IP-MS enrichment of TSC2 (LFQ intensity): TSC2 was enriched 1134-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and TSC2 antibody (Part No. MA5-15004). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.