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Western blot analysis of TSC2 was performed by loading 20ug of two independent 293T cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in PBST for 1 hour at room temperature. The membrane was probed with a TSC2 monoclonal antibody (Product # MA5-15004) at a dilution of 1:1000 at 4°C overnight, washed in PBST, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:2000 for 1 hour at room temperature. As a loading control, the same lysates were probed with an antibody against alpha-Tubulin. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide from the amino terminus of human tuberin|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/ml BSA|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
OTTHUMP00000198394; protein phosphatase 1, regulatory subunit 160; tuberin; tuberous sclerosis 2 protein
LAM; PPP1R160; TSC2; TSC4