Immunofluorescence analysis of alpha Tubulin antibody was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin Rabbit Polyclonal Antibody (PA522060) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Fruit fly, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contain a sequence corresponding to a region within amino acids 37 and 276 (Q71U36) of alpha Tubulin 1A|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1,000 - 1:5,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-22060 targets Tubulin alpha 1A in ICC, IF, IHC (P), and WB applications and shows reactivity with Drosophila, Human, Rat and mouse samples.
The PA5-22060 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 37 and 276 (Q71U36) of alpha Tubulin 1A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Gustatory Signaling Pathway and Bitter Taste Receptors Affect the Development of Obesity and Adipocyte Metabolism in Mice.
PA5-22060 was used in western blot to investigate the role of the gustatory signaling pathway in diet-induced obesity
|Avau B,Bauters D,Steensels S,Vancleef L,Laermans J,Lesuisse J,Buyse J,Lijnen HR,Tack J,Depoortere I||PloS one (10:null)||2015|