Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunohistochemistry analysis of Tubulin alpha showing staining in the cytoplasm of paraffin-embedded human lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Tubulin alpha Rabbit Polyclonal Antibody (PA516891) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Gerbil , Guinea pig , Human , Rat , Amphibian , Cattle , Mouse , Porcine , Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from aa 426-450 of human tubulin alpha|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16891 targets Tubulin alpha in immunohistochemistry (paraffin) and Western blot applications and shows reactivity with Bovine, Chicken, amphibian, Gerbil, Guinea Pig, Human, mouse, Porcine, and Rat samples.
The PA5-16891 immunogen is a synthetic peptide derived from aa 426-450 of human tubulin alpha.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
LIS3, Tuba1, BOS_5053, TUBA3, TUBA1, TUBA6, Tuba-1, B-ALPHA-1
hum-a-tub1, hum-a-tub2, tubulin B-alpha-1, tubulin alpha-1A chain, tubulin alpha-3 chain, tubulin, alpha, brain-specific, Talpha1 alpha-tubulin, alpha-tubulin 1, alpha-tubulin isotype M-alpha-1, tubulin alpha-1 chain, tubulin, alpha 1, alpha-tubulin ubiquitous, tubulin K-alpha-1, tubulin alpha-1B chain, tubulin alpha-ubiquitous chain, alpha-tubulin 3, tubulin, alpha 3, alpha, brain-specific