Immunofluorescence analysis of Tubulin alpha was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tubulin alpha Rabbit Polyclonal Antibody (Product # PA5-16891) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Amphibian, Bovine, Chicken, Gerbil, Guinea pig, Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from aa 426-450 of human tubulin alpha|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:50-1:500|
PA5-16891 targets Tubulin alpha in immunohistochemistry (paraffin) and Western blot applications and shows reactivity with Bovine, Chicken, amphibian, Gerbil, Guinea Pig, Human, mouse, Porcine, and Rat samples.
The PA5-16891 immunogen is a synthetic peptide derived from aa 426-450 of human tubulin alpha.
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
alpha; alpha 3; alpha-tubulin 1; alpha-tubulin 3; alpha-tubulin isotype M-alpha-1; alpha-tubulin ubiquitous; brain-specific; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; tubulin; tubulin alpha-1 chain; tubulin alpha-1A chain; tubulin alpha-1B chain; tubulin alpha-3 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1a; tubulin, alpha, brain-specific
B-ALPHA-1; BOS_5053; LIS3; Tuba-1; TUBA1; TUBA1A; TUBA3; TUBA6