|Published species reactivity||Human|
|Host / Isotype||E.coli|
|Storage buffer||4% BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 2 publications below|
The human TNF? Standard is calibrated against WHO TNF? Reference Standard lot #87/650. 1 Thermo pg = 1 WHO pg = 0.04 WHO units.
Lyophilized product is shipped on cold packs. Stable until expiration if stored desiccated at 2-8°C. Reconstituted cytokine standards should be stored in aliquots at -70°C. Each aliquot should be used only once and discarded afterwards. Stability of reconstituted and frozen cytokine standards may vary. Controls should be run with each standard curve.
Sufficient For: ELISA standard curve with replicates or as a control in Western blots.
2500 pg/ml when reconstituted at the recommend volume
Reconstitute with distilled water at volume indicated on label. Use or freeze product within 1 hour of reconstitution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effect of age on surface molecules and cytokine expression in human dendritic cells.
STNFA was used in ELISA to investigate the immunosenescence of human dendritic cells
|Ciaramella A,Spalletta G,Bizzoni F,Salani F,Caltagirone C,Bossù P||Cellular immunology (269:82)||2011|
Influence of synthetic antiendotoxin peptides on lipopolysaccharide (LPS) recognition and LPS-induced proinflammatory cytokine responses by cells expressing membrane-bound CD14.
STNFA was used in ELISA to study the endotoxin-neutralizing activities of two synthetic anti-endotoxin peptides, polymixin B, and an LPS core-specific monoclonal antibody
|Iwagaki A,Porro M,Pollack M||Infection and immunity (68:1655)||2000|