Invitrogen

Tyrosine Hydroxylase Polyclonal Antibody

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Antibody Testing Data (2)
Published Figures (2)

Tyrosine Hydroxylase Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Tyrosine Hydroxylase Antibody (P21962) in IHC (F)

Immunofluorescence analysis of DA neurons using anti-tyrosine hydroxylase (TH) antibody. H9 ESCs were differentiated with PSC Dopaminergic neuron differentiation kit. H9 ESCs were specified to become midbrain floor plate (FP) progenitors which were further expanded and cryopreserved. Recovered FP progenitors were then matured for additional 14 days. Expression of TH was labeled with 1st antibody, anti-TH (P... View More

Tyrosine Hydroxylase Antibody in Western Blot (WB)

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Product Details

P21962

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Fruit fly, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539844

Target Information

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mutant β₁-adrenergic receptor improves REM sleep and ameliorates tau accumulation in a mouse model of tauopathy.

Proceedings of the National Academy of Sciences of the United States of America

Dong Q,Ptáček LJ,Fu YH

P21962 was used in Immunohistochemistry-immunofluorescence to test the effect of another familial natural short sleep (FNSS) gene variant, Adrb1-A187V, by crossing mice with this mutation onto the PS19 background.

Tue Apr 11 00:00:00 EDT 2023

Intraperitoneal Administration of Forskolin Reverses Motor Symptoms and Loss of Midbrain Dopamine Neurons in PINK1 Knockout Rats.

Journal of Parkinson's disease

Vazquez-Mayorga E,Grigoruta M,Dagda R,Martinez B,Dagda RK

P21962 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to suggest that Forskolin is a promising disease-modifying therapeutic alternative for PD and is superior to Levodopa because it confers long-lasting therapeutic effects.

Wed Apr 13 00:00:00 EDT 2022

Diurnal rhythms in cholinergic modulation of rapid dopamine signals and associative learning in the striatum.

Cell reports

Stowe TA,Pitts EG,Leach AC,Iacino MC,Niere F,Graul B,Raab-Graham KF,Yorgason JT,Ferris MJ

P21962 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to conclude that the rapid dopamine signaling rhythm is mediated by a diurnal rhythm in CIN activity, which influences learning and motivated behaviors across the time of day.

Tue Apr 05 00:00:00 EDT 2022

Dorsal striatal dopamine induces fronto-cortical hypoactivity and attenuates anxiety and compulsive behaviors in rats.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

Casado-Sainz A,Gudmundsen F,Baerentzen SL,Lange D,Ringsted A,Martinez-Tejada I,Medina S,Lee H,Svarer C,Keller SH,Schain M,Kjaerby C,Fisher PM,Cumming P,Palner M

P21962 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to investigate effects of targeted dopamine activation on CSTC circuit function, especially in fronto-cortical regions.

Sat Jan 01 00:00:00 EST 2022

Integrated age-related immunohistological changes occur in human olfactory epithelium and olfactory bulb.

The Journal of comparative neurology

Fitzek M,Patel PK,Solomon PD,Lin B,Hummel T,Schwob JE,Holbrook EH

P21962 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to characterize changes associated with age in the peripheral olfactory system, by in-depth analysis of the OE and its neuronal projections onto the olfactory bulb (OB) as a function of age.

Mon Aug 01 00:00:00 EDT 2022

Insulin Bidirectionally Alters NAc Glutamatergic Transmission: Interactions between Insulin Receptor Activation, Endogenous Opioids, and Glutamate Release.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fetterly TL,Oginsky MF,Nieto AM,Alonso-Caraballo Y,Santana-Rodriguez Z,Ferrario CR

P21962 was used in Western Blot to understand how insulin influences excitatory transmission in the nucleus accumbens.

Wed Mar 17 00:00:00 EDT 2021

Insulin Bidirectionally Alters NAc Glutamatergic Transmission: Interactions between Insulin Receptor Activation, Endogenous Opioids, and Glutamate Release.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fetterly TL,Oginsky MF,Nieto AM,Alonso-Caraballo Y,Santana-Rodriguez Z,Ferrario CR

P21962 was used in Western Blot to understand how insulin influences excitatory transmission in the nucleus accumbens.

Wed Mar 17 00:00:00 EDT 2021

Ventral Tegmental Dopamine Neurons Control the Impulse Vector during Motivated Behavior.

Current biology : CB

Hughes RN,Bakhurin KI,Petter EA,Watson GDR,Kim N,Friedman AD,Yin HH

P21962 was used in Immunohistochemistry (PFA fixed) to investigate the association of dopamine neurone activity and the impulse vector exerted by the mice.

Mon Jul 20 00:00:00 EDT 2020

Dexmedetomidine Activation of Dopamine Neurons in the Ventral Tegmental Area Attenuates the Depth of Sedation in Mice.

Anesthesiology

Qiu G,Wu Y,Yang Z,Li L,Zhu X,Wang Y,Sun W,Dong H,Li Y,Hu J

P21962 was used in Immunohistochemistry (PFA fixed) to hypothesize that dexmedetomidine increases the activity of dopaminergic neurons in the ventral tegmental area, and that this action contributes to the unique sedative properties of dexmedetomidine.

Sat Aug 01 00:00:00 EDT 2020

Psychological Stress Phenocopies Brain Mitochondrial Dysfunction and Motor Deficits as Observed in a Parkinsonian Rat Model.

Molecular neurobiology

Grigoruţă M,Martínez-Martínez A,Dagda RY,Dagda RK

P21962 was used in Western Blotting to suggest that psychological distress can phenocopy several aspects of PD neuropathology, disrupt brain energy production, as well as induce ataxia-like behavior.

Wed Apr 01 00:00:00 EDT 2020

Absence of Glia Maturation Factor Protects from Axonal Injury and Motor Behavioral Impairments after Traumatic Brain Injury.

Experimental neurobiology

Selvakumar GP,Ahmed ME,Iyer SS,Thangavel R,Kempuraj D,Raikwar SP,Bazley K,Wu K,Khan A,Kukulka K,Bussinger B,Zaheer S,Burton C,James D,Zaheer A

P21962 was used in Western Blotting to provide evidence that PD-associated pathology progression can be initiated upon induction of TBI.

Tue Jun 30 00:00:00 EDT 2020

Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.

eNeuro

Larsson M,Broman J

P21962 was used in Immunohistochemistry-immunofluorescence to investigate the nature of the central termination of low-threshold mechanosensitive C fibres in the rodent dorsal horn.

Tue May 21 00:00:00 EDT 2019

The Neuroregenerative Capacity of Olfactory Stem Cells Is Not Limitless: Implications for Aging.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Child KM,Herrick DB,Schwob JE,Holbrook EH,Jang W

Published figure using Tyrosine Hydroxylase polyclonal antibody (Product # P21962) in Western Blot

Wed Aug 01 00:00:00 EDT 2018

The Neuroregenerative Capacity of Olfactory Stem Cells Is Not Limitless: Implications for Aging.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Child KM,Herrick DB,Schwob JE,Holbrook EH,Jang W

Published figure using Tyrosine Hydroxylase polyclonal antibody (Product # P21962) in Western Blot

Wed Aug 01 00:00:00 EDT 2018

Wakefulness Is Promoted during Day Time by PDFR Signalling to Dopaminergic Neurons in Drosophila melanogaster.

eNeuro

Potdar S,Sheeba V

P21962 was used in Immunocytochemistry-immunoflourescence to identify a novel circadian clock pathway that mediates wake promotion specifically during day time.

Wed Feb 27 00:00:00 EST 2019

Role of the IL-1 Pathway in Dopaminergic Neurodegeneration and Decreased Voluntary Movement.

Molecular neurobiology

Stojakovic A,Paz-Filho G,Arcos-Burgos M,Licinio J,Wong ML,Mastronardi CA

P21962 was used in immunohistochemistry to elucidate role of the IL-1 pathway on dopaminergic neurodegeneration and motor skills during aging

Tue Aug 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

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Gene Aliases: DYT14; DYT5b; TH; The; TYH

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UniProt ID: (Human) P07101, (Rat) P04177

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Entrez Gene ID: (Human) 7054, (Rat) 25085

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Function(s)

tyrosine 3-monooxygenase activity protein binding ferrous iron binding ferric iron binding amino acid binding oxygen binding enzyme binding protein domain specific binding tetrahydrobiopterin binding dopamine binding monooxygenase activity

Process(es)

response to hypoxia synaptic transmission, dopaminergic response to amphetamine heart morphogenesis dopamine biosynthetic process from tyrosine fatty acid metabolic process sphingolipid metabolic process heart development visual perception sensory perception of sound learning memory mating behavior locomotory behavior regulation of heart contraction response to water deprivation response to light stimulus response to herbicide response to salt stress anatomical structure morphogenesis organ morphogenesis response to zinc ion multicellular organism aging response to activity aminergic neurotransmitter loading into synaptic vesicle glycoside metabolic process phthalate metabolic process cerebral cortex development response to nutrient levels response to estradiol response to lipopolysaccharide isoquinoline alkaloid metabolic process social behavior cellular response to drug response to isolation stress response to immobilization stress neurotransmitter biosynthetic process terpene metabolic process dopamine biosynthetic process epinephrine biosynthetic process norepinephrine biosynthetic process catecholamine biosynthetic process eye photoreceptor cell development circadian sleep/wake cycle eating behavior response to peptide hormone pigmentation response to ethanol response to ether response to pyrethroid embryonic camera-type eye morphogenesis response to corticosterone response to electrical stimulus phytoalexin metabolic process oxidation-reduction process cellular response to manganese ion cellular response to nicotine cellular response to glucose stimulus cellular response to growth factor stimulus response to metal ion response to organic cyclic compound response to insecticide response to nicotine response to drug response to steroid hormone cognition protein homotetramerization response to growth factor cellular response to alkaloid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Tyrosine Hydroxylase Polyclonal Antibody

Tyrosine Hydroxylase Antibody (P21962) in IHC (F)

Product Details

P21962

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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