|Tested species reactivity||Many|
|Published species reactivity||Dog, Mouse, Not Applicable|
|Host / Isotype||Sheep / IgG|
|Immunogen||SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:1000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for the ~60 kDa Tyrosine Hydroxylase in Western blots of rat brain extracts.
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson and quote;s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Loss of the transcription factor Meis1 prevents sympathetic neurons target-field innervation and increases susceptibility to sudden cardiac death.
PA1-4679 was used in immunohistochemistry - frozen section to determine how the loss of a transcription factor, Meis1, prevents innervation of sympathetic neurons and increases the likelihood of sudden cardiac death
|Bouilloux F,Thireau J,Ventéo S,Farah C,Karam S,Dauvilliers Y,Valmier J,Copeland NG,Jenkins NA,Richard S,Marmigère F||eLife (5:null)||2016|
Abnormal sinoatrial node development resulting from disturbed vascular endothelial growth factor signaling.
PA1-4679 was used in immunohistochemistry to study the role of vascular endothelial growth factor in sinoatrial node development.
|Calkoen EE,Vicente-Steijn R,Hahurij ND,van Munsteren CJ,Roest AA,DeRuiter MC,Steendijk P,Schalij MJ,Gittenberger-de Groot AC,Blom NA,Jongbloed MR||International journal of cardiology (183:249)||2015|
Astroglial heme oxygenase-1 and the origin of corpora amylacea in aging and degenerating neural tissues.
PA1-4679 was used in immunohistochemistry to study the role of astroglial heme oxygenase-1 in the generation of corpora amylacea in cultured cells and in the aging brain and the therapeutic implications for neurodegenerative diseases
|Song W,Zukor H,Liberman A,Kaduri S,Arvanitakis Z,Bennett DA,Schipper HM||Experimental neurology (254:78)||2014|
Catheter-based renal sympathetic denervation significantly inhibits atrial fibrillation induced by electrical stimulation of the left stellate ganglion and rapid atrial pacing.
PA1-4679 was used in immunohistochemistry to study the effects of renal sympathetic denervation on atrial fibrillation in a canine hyper-sympathetic tone model
|Hou Y,Hu J,Po SS,Wang H,Zhang L,Zhang F,Wang K,Zhou Q||PloS one (8:null)||2013|