Invitrogen

Tyrosine Hydroxylase Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

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Tyrosine Hydroxylase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of Tyrosine Hydroxylase in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Tyrosine Hydroxylase Polyclonal Antibody (Product # PA5-118306, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.

Tyrosine Hydroxylase Antibody in Western Blot (WB) — Western Blot using Tyrosine Hydroxylase Polyclonal Antibody (Product # PA5-118306) at 1:500 dilution. Lane A: PC12 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 59 kDa.

Tyrosine Hydroxylase Antibody in Immunoprecipitation (IP) — Tyrosine Hydroxylase Immunoprecipitation using: Lane A: 0.5 mg PC-3 Whole Cell Lysate 0.5 µL with Tyrosine Hydroxylase Polyclonal Antibody (Product # PA5-118306) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: Tyrosine Hydroxylase Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 99 kDa.

Product Details

PA5-118306

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunocytochemistry (ICC/IF)

1:1,500-1:50,000

Immunoprecipitation (IP)

0.2-1 μL/mg of lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the C-terminus of the Human Tyrosine Hydroxylase/TH

View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2902901

Target Information

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

Gene Aliases: DYT14; DYT5b; TH; TYH

UniProt ID: (Human) P07101

Entrez Gene ID: (Human) 7054

Function(s)

response to hypoxia synaptic transmission, dopaminergic response to amphetamine heart morphogenesis dopamine biosynthetic process from tyrosine fatty acid metabolic process sphingolipid metabolic process heart development visual perception sensory perception of sound learning memory mating behavior locomotory behavior regulation of heart contraction response to water deprivation response to light stimulus response to herbicide response to salt stress anatomical structure morphogenesis organ morphogenesis response to zinc ion multicellular organism aging response to activity aminergic neurotransmitter loading into synaptic vesicle glycoside metabolic process phthalate metabolic process cerebral cortex development response to nutrient levels response to estradiol response to lipopolysaccharide isoquinoline alkaloid metabolic process social behavior cellular response to drug response to isolation stress response to immobilization stress neurotransmitter biosynthetic process terpene metabolic process dopamine biosynthetic process epinephrine biosynthetic process norepinephrine biosynthetic process catecholamine biosynthetic process eye photoreceptor cell development circadian sleep/wake cycle eating behavior response to peptide hormone pigmentation response to ethanol response to ether response to pyrethroid embryonic camera-type eye morphogenesis response to corticosterone response to electrical stimulus phytoalexin metabolic process oxidation-reduction process cellular response to manganese ion cellular response to nicotine cellular response to glucose stimulus cellular response to growth factor stimulus

Process(es)

tyrosine 3-monooxygenase activity protein binding ferrous iron binding ferric iron binding amino acid binding oxygen binding enzyme binding protein domain specific binding tetrahydrobiopterin binding dopamine binding