Invitrogen

Tyrosine Hydroxylase Polyclonal Antibody

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Tyrosine Hydroxylase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of Tyrosine Hydroxylase in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Tyrosine Hydroxylase Polyclonal Antibody (Product # PA5-118306, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.

Tyrosine Hydroxylase Antibody in Western Blot (WB) — Western Blot using Tyrosine Hydroxylase Polyclonal Antibody (Product # PA5-118306) at 1:500 dilution. Lane A: PC12 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 59 kDa.

Tyrosine Hydroxylase Antibody in Immunoprecipitation (IP) — Tyrosine Hydroxylase Immunoprecipitation using: Lane A: 0.5 mg PC-3 Whole Cell Lysate 0.5 µL with Tyrosine Hydroxylase Polyclonal Antibody (Product # PA5-118306) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: Tyrosine Hydroxylase Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 99 kDa.

Product Details

PA5-118306

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	1:1,500-1:50,000	-
Immunoprecipitation (IP)	0.2-1 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to the C-terminus of the Human Tyrosine Hydroxylase/TH View antigen
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

Gene Aliases: DYT14; DYT5b; TH; TYH

UniProt ID: (Human) P07101

Entrez Gene ID: (Human) 7054

Function(s)