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Invitrogen
Tyrosine Hydroxylase Polyclonal Antibody
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FIGURE: 1 / 5
Tyrosine Hydroxylase Antibody (PA5-18372) in ICC/IF
Immunofluorescence analysis of Tyrosine Hydroxylase was performed using 70% confluent log phase PC-12 cells treated with Dexamethasone (5 µg/mL, 6 days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Tyrosine Hydroxylase Polyclonal Antibody (Product # ... View More
Product Details
PA5-18372
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (VQDELDTLAHAL) corresponding to the C-terminus amino acids of TH (aa 513-525).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation, Antigen affinity chromatography
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with canine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.
Target Information
Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aromatic amino acid hydroxylase; dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase (EC 1.14.16.2); tyrosine hydroxylase type 4 (EC 1.14.16.2); unnamed protein product
Gene Aliases: DYT14; DYT5b; TH; TYH
UniProt ID: (Human) P07101
Entrez Gene ID: (Human) 7054
monooxygenase activity
tyrosine 3-monooxygenase activity
iron ion binding
protein binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
enzyme binding
identical protein binding
metal ion binding
response to hypoxia
synaptic transmission, dopaminergic
heart morphogenesis
dopamine biosynthetic process from tyrosine
heart development
visual perception
learning
memory
mating behavior
locomotory behavior
regulation of heart contraction
aromatic amino acid family metabolic process
anatomical structure morphogenesis
organ morphogenesis
dopamine biosynthetic process
epinephrine biosynthetic process
norepinephrine biosynthetic process
catecholamine biosynthetic process
serotonin biosynthetic process
eye photoreceptor cell development
eating behavior
pigmentation
response to ethanol
embryonic camera-type eye morphogenesis
cognition
hyaloid vascular plexus regression
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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