Immunofluorescence analysis of DA neurons using anti-tyrosine hydroxylase (TH) antibody. H9 ESCs were differentiated with PSC Dopaminergic neuron differentiation kit (Life Technologies). H9 ESCs were specified to become midbrain floor plate (FP) progenitors which were further expanded and cryopreserved. Recovered FP progenitors were then matured for additional 14 days. Expression of TH was labeled with 1st antibody, anti-TH (Cat. No. P21962) followed by 2nd antibody AlexaFluor488 goat anti-rabbit (Cat. No. A21206, green). Nuclear DNA was stained with DAPI (blue).
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/ml BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters (dopamine, epinephrine, and norepinephrine). It is responsible for the conversion of L-tyrosine to L-dopa in the catecholamine synthesis pathway
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