|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 139 and 282 of Human U1A|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-27474 targets U1A in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-27474 immunogen is recombinant fragment corresponding to a region within amino acids 139 and 282 of Human U1A.
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5 and apos; splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SRSF1 and hnRNP H antagonistically regulate splicing of COLQ exon 16 in a congenital myasthenic syndrome.
PA5-27474 was used in western blot to characterize acetylcholinesterase mutations.
|Rahman MA,Azuma Y,Nasrin F,Takeda J,Nazim M,Bin Ahsan K,Masuda A,Engel AG,Ohno K||Scientific reports (5:null)||2015|