Immunofluorescent analysis of U1SNRNPBP showing staining in the nucleus of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a U1SNRNPBP polyclonal antibody (Product # PA5-31496) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 45 and 246 of Human U1SNRNPBP|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31496 targets U1SNRNPBP in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-31496 immunogen is recombinant fragment corresponding to a region within amino acids 45 and 246 of Human U1SNRNPBP.
The protein encoded by this gene is a homolog of U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal is rich in Arg/Asp and Arg/Glu dipeptides; a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. This gene is differentially expressed in a variety of human tissues. Alternative splicing results in multiple transcript variants encoding distinct proteins.
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