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Immunofluorescent analysis of U1SNRNPBP showing staining in the nucleus of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a U1SNRNPBP polyclonal antibody (Product # PA5-31496) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 45 and 246 of Human U1SNRNPBP|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31496 targets U1SNRNPBP in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-31496 immunogen is recombinant fragment corresponding to a region within amino acids 45 and 246 of Human U1SNRNPBP.
The protein encoded by this gene is a homolog of U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal is rich in Arg/Asp and Arg/Glu dipeptides; a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. This gene is differentially expressed in a variety of human tissues. Alternative splicing results in multiple transcript variants encoding distinct proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HM-1; protein HM-1; small nuclear ribonucleoprotein 35kDa (U11/U12); small nuclear ribonucleoprotein, U11/U12 35kDa subunit; U1 snRNP binding protein homolog; U1 snRNP-binding protein homolog; U1-snRNP binding protein homolog; U11/U12 small nuclear ribonucleoprotein 35 kDa protein; U11/U12 snRNP 35 kDa protein; U11/U12 snRNP 35K; U11/U12-35K; U1SNRNPBP
6330548G22Rik; HM-1; HM1; SNRNP35; U1SNRNPBP