For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi.
Protein Aliases: 60S ribosomal protein L40; CEP52; HUB L40; Large ribosomal subunit protein eL40; MGC127041; Ubiquitin; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin carboxyl extension protein 52; ubiquitin-52 amino acid fusion protein; Ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52
Gene Aliases: CEP52; HUBCEP52; L40; RPL40; UBA52; UBCEP2
UniProt ID: (Human) P62987
Entrez Gene ID: (Human) 7311