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Immunofluorescent staining of HeLa cells using PA5-19675, anti-UBC6e antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 5 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human UBC6e.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19675 targets UBC6e in IF and WB applications and shows reactivity with Human samples.
The PA5-19675 immunogen is synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human UBC6e.
PA5-19675 detects UBC6e which has a predicted molecular weight of approximately 37 kDa.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CGI-76, HSPC153, HSPC205, HSU93243, MGC12555, NCUBE-1, NCUBE1, Ubc6p, HSUBC6e; E2 ubiquitin-conjugating enzyme J1; HSUBC6e; non-canonical ubiquitin-conjugating enzyme 1; non-canonical ubquitin conjugating enzyme 1; ubiquitin conjugating enzyme E2, J1; ubiquitin-conjugating enzyme E2 J1; ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast); ubiquitin-conjugating enzyme E2, J1, U; yeast ubiquitin-conjugating enzyme UBC6 homolog E
CGI-76; HSPC153; HSPC205; HSU93243; NCUBE-1; NCUBE1; UBC6; UBC6E; Ubc6p; UBE2J1