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Immunofluorescent analysis of UBE2C showing staining in the cytoplasm and nucleus of A549 cells. A549 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a UBE2C polyclonal antibody (Product # PA5-27223) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 179 of Human UBE2C|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27223 targets UBE2C in WB applications and shows reactivity with Human and mouse samples.
The PA5-27223 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 179 of Human UBE2C.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
(E3-independent) E2 ubiquitin-conjugating enzyme C; cyclin-selective ubiquitin carrier protein; dJ447F3.2; E2 ubiquitin-conjugating enzyme C; mitotic-specific ubiquitin-conjugating enzyme; UBCH10; ubiquitin carrier protein C; ubiquitin carrier protein E2-C; ubiquitin conjugating enzyme E2C; ubiquitin-conjugating enzyme E2 C; ubiquitin-conjugating enzyme E2C; ubiquitin-protein ligase C
1110015A16Rik; D2Ertd695e; dJ447F3.2; UBCH10; UBE2C