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UBE2D2 Antibody (CF806599) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D. Positive lysates LY418757 (100 µg) and LC418757 (20 µg) can be purchased separately from OriGene.
Product Details
CF806599
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI3H1
Immunogen
Full length human recombinant protein of human UBE2D2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (E3-independent) E2 ubiquitin-conjugating enzyme D2; E2 ubiquitin-conjugating enzyme D2; p53-regulated ubiquitin-conjugating enzyme 1; Ubiquitin carrier protein D2; ubiquitin conjugating enzyme E2D 2; Ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); Ubiquitin-protein ligase D2
Gene Aliases: E2(17)KB2; PUBC1; UBC4; UBC4/5; UBC5B; UBCH4; UBCH5B; UBE2D2
UniProt ID: (Human) P62837
Entrez Gene ID: (Human) 7322
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
cellular protein modification process
ubiquitin-dependent protein catabolic process
protein ubiquitination
TRIF-dependent toll-like receptor signaling pathway
Fc-epsilon receptor signaling pathway
T cell receptor signaling pathway
protein autoubiquitination
regulation of transcription from RNA polymerase II promoter in response to hypoxia
protein K48-linked ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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