For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Protein Aliases: E2 ubiquitin-conjugating enzyme D4; HBUCE1; Ubiquitin carrier protein D4; ubiquitin conjugating enzyme E2D 4 (putative); Ubiquitin-conjugating enzyme E2 D4; ubiquitin-conjugating enzyme E2D 4 (putative); ubiquitin-conjugating enzyme HBUCE1; Ubiquitin-protein ligase D4
Gene Aliases: HBUCE1; UBCH5D; UBE2D4
UniProt ID: (Human) Q9Y2X8
Entrez Gene ID: (Human) 51619
Molecular Function: ubiquitin-protein ligase