|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human Ubc13|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
STAT3 restrains RANK- and TLR4-mediated signalling by suppressing expression of the E2 ubiquitin-conjugating enzyme Ubc13.
37-1100 was used in western blot to elucidate the mechanism by which STAT3 controls NF-kapaB signaling in innate immune cells.
|Zhang H,Hu H,Greeley N,Jin J,Matthews AJ,Ohashi E,Caetano MS,Li HS,Wu X,Mandal PK,McMurray JS,Moghaddam SJ,Sun SC,Watowich SS||Nature communications (5:null)||2014|
A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.
37-1100 was used in western blot to identify E2s that regulate the activation, translocation, and enzymatic functions of Parkin during mitochondrial quality control
|Fiesel FC,Moussaud-Lamodière EL,Ando M,Springer W||Journal of cell science (127:3488)||2014|
A small-molecule inhibitor of UBE2N induces neuroblastoma cell death via activation of p53 and JNK pathways.
37-1100 was used in western blot to study the effect of a UBE2N inhibitor on neuroblastoma and its mechanism.
|Cheng J,Fan YH,Xu X,Zhang H,Dou J,Tang Y,Zhong X,Rojas Y,Yu Y,Zhao Y,Vasudevan SA,Zhang H,Nuchtern JG,Kim ES,Chen X,Lu F,Yang J||Cell death and disease (5:null)||2014|
|Not Applicable||Not Cited||
USP21 negatively regulates antiviral response by acting as a RIG-I deubiquitinase.
37-1100 was used in western blot to report a role for USP21 in the negative regulation of antiviral response through deubiquitinating RIG-I
|Fan Y,Mao R,Yu Y,Liu S,Shi Z,Cheng J,Zhang H,An L,Zhao Y,Xu X,Chen Z,Kogiso M,Zhang D,Zhang H,Zhang P,Jung JU,Li X,Xu G,Yang J||The Journal of experimental medicine (211:313)||2014|
E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1.
37-1100 was used in western blot to assess the deubiquitinating activity of OTUB1 regulated by E2 ubiquitin-conjugating enzymes
|Wiener R,DiBello AT,Lombardi PM,Guzzo CM,Zhang X,Matunis MJ,Wolberger C||Nature structural and molecular biology (20:1033)||2013|
Inhibition of proliferation and survival of diffuse large B-cell lymphoma cells by a small-molecule inhibitor of the ubiquitin-conjugating enzyme Ubc13-Uev1A.
37-1100 was used in western blot to identify and characterize compounds that inhibit NF-κB activation in diffuse large B-cell lymphoma cells.
|Pulvino M,Liang Y,Oleksyn D,DeRan M,Van Pelt E,Shapiro J,Sanz I,Chen L,Zhao J||Blood (120:1668)||2012|
Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress.
37-1100 was used in western blot to study the role of ZRANB3 translocase during replication.
|Ciccia A,Nimonkar AV,Hu Y,Hajdu I,Achar YJ,Izhar L,Petit SA,Adamson B,Yoon JC,Kowalczykowski SC,Livingston DM,Haracska L,Elledge SJ||Molecular cell (47:396)||2012|
|Mouse||Not Cited||Regulation of hematopoiesis by the K63-specific ubiquitin-conjugating enzyme Ubc13.||Wu X,Yamamoto M,Akira S,Sun SC||Proceedings of the National Academy of Sciences of the United States of America (106:20836)||2009|
RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
37-1100 was used in western blot to investigate the potential role of the proteasome in checkpoint activation and ATM/ATR signaling in response to UV light-induced DNA damage
|Sakasai R,Tibbetts R||The Journal of biological chemistry (283:13549)||2008|
|Not Applicable||Not Cited||
The human T-cell leukemia virus type 1 Tax oncoprotein requires the ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation.
37-1100 was used in immunoprecipitation and western blot to study the requirement of ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation by human T-cell leukemia virus 1 tax oncoprotein
|Shembade N,Harhaj NS,Yamamoto M,Akira S,Harhaj EW||Journal of virology (81:13735)||2007|
|Not Applicable||Not Cited||
Cutting Edge: Pivotal function of Ubc13 in thymocyte TCR signaling.
37-1100 was used in western blot to determine the function of Ubc13 in T cell receptor-mediated signaling and responses
|Yamamoto M,Sato S,Saitoh T,Sakurai H,Uematsu S,Kawai T,Ishii KJ,Takeuchi O,Akira S||Journal of immunology (Baltimore, Md. : 1950) (177:7520)||2006|
|Human||Not Cited||TIFA activates IkappaB kinase (IKK) by promoting oligomerization and ubiquitination of TRAF6.||Ea CK,Sun L,Inoue J,Chen ZJ||Proceedings of the National Academy of Sciences of the United States of America (101:15318)||2004|
|Human||Not Cited||Ubiquitination and translocation of TRAF2 is required for activation of JNK but not of p38 or NF-kappaB.||Habelhah H,Takahashi S,Cho SG,Kadoya T,Watanabe T,Ronai Z||The EMBO journal (23:322)||2004|
The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.
37-1100 was used in immunoprecipitation and western blot to show that RNF168 is mutated in individuals with RIDDLE syndrome
|Stewart GS,Panier S,Townsend K,Al-Hakim AK,Kolas NK,Miller ES,Nakada S,Ylanko J,Olivarius S,Mendez M,Oldreive C,Wildenhain J,Tagliaferro A,Pelletier L,Taubenheim N,Durandy A,Byrd PJ,Stankovic T,Taylor AM,Durocher D||Cell (136:420)||2009|
|Noncanonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly.||Huen MS,Huang J,Yuan J,Yamamoto M,Akira S,Ashley C,Xiao W,Chen J||Molecular and cellular biology (28:6104)||2008|