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Immunofluorescent analysis of UBE2N showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a UBE2N polyclonal antibody (Product # PA5-29687) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 131 of Human UBE2N|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29687 targets UBE2N in WB applications and shows reactivity with Human and mouse samples.
The PA5-29687 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 131 of Human UBE2N.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
bendless-like ubiquitin conjugating enzyme; bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; epididymis secretory protein Li 71; UBC13; UbcH-ben; ubiquitin carrier protein N; ubiquitin conjugating enzyme E2N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yea; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog; ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-protein ligase N; yeast UBC13 homolog; yeast)
1500026J17Rik; AL022654; BB101821; BLU; HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN; UBC13; UBE2N