Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1-3 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
This UBE2V2 antibody was raised against a peptide corresponding to amino acid residues 12-28 of human UBE2V2. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
See Data Sheet |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.5, with 0.2% gelatin |
Contains |
0.05% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2793196 |
The differentiation and activation of monocytes (MO) and monocytic cells is modulated by 1alpha,25-dihydroxyvitamin D3 (Vitamin D3). Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3. This cDNA encodes a putative 16.5 kD protein. The product of this gene has structural homology with yeast MMS2p and human CROC-1), which defines a structurally and functionally conserved Ubc-like protein family. This protein was called Ubiquitin E2 variant (UBE2V2) or MMS2 (methyl methanesulfonate sensitive 2). The yeast UEV protein, Mms2, is required together with Ubc13 for RAD6/RAD18-dependent postreplicative DNA repair in yeast. These proteins also play a central role in the assembly of K63-linked polyubiquitin chains (4,5) UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the Mms2/Ubc13 heterodimer is necessary for DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1 alpha,25-dihydroxyvitamin D3-inducible; DDVit 1; EDAF-1; EDPF-1; enterocyte differentiation promoting factor; Enterocyte differentiation-associated factor 1; enterocyte differentiation-associated factor EDAF-1; Enterocyte differentiation-promoting factor 1; meth; methyl methanesulfonate sensitive 2, S. cerevisiae, homolog of; MMS2 homolog; ubiquitin conjugating enzyme E2 variant 2; Ubiquitin-conjugating enzyme E2 variant 2; Vitamin D3-inducible protein
Gene Aliases: DDVit-1; DDVIT1; EDAF-1; EDPF-1; EDPF1; MMS2; UBE2V2; UEV-2; UEV2
UniProt ID: (Human) Q15819
Entrez Gene ID: (Human) 7336
Molecular Function:
ubiquitin-protein ligase
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