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UBE3C Antibody (A304-122A) in WB
Detection of human UBE3C by western blot. Samples: Whole cell lysate (50 µg) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBE3C antibody (Product # A304-122A) (lot A304-122A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A304-122A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 350 to 400 of Human Ubiquitin Protein Ligase E3C.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HECT-type ubiquitin transferase E3C; HectH2; Homologous to E6AP carboxyl terminus homologous protein 2; RAUL; RTA-associated ubiquitin ligase; ubiquitin-protein isopeptide ligase (E3); Ubiquitin-protein ligase E3C
Gene Aliases: HECTH2; KIAA0010; KIAA10; UBE3C
UniProt ID: (Human) A4D235
Entrez Gene ID: (Human) 9690
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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