Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 (MA1-46079) (green) and rabbit antibody to neurofilament NF-M, (NB300-133), (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.
|Tested species reactivity||Bovine, Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant full length human UCHL1 purified from E. coli.|
|Contains||10mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: bovine brain whole cell homogenate, SHSY-5Y or HEK 293 cell extract, and rat spinal cord/peripheral nerve homogenate, antigen standard for UCHL1 (transient overexpression lysate).
Ubiquitin C-terminal hydrolase 1 (UCHL1) was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. The protein is extremely abundant representing 1-2% of total
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
epididymis luminal protein 117; epididymis secretory protein Li 53; neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP95; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; ubiquitin thiolesterase; UCH-L1
BOS_6576; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1; UCHL1