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Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 (MA1-46079) (green) and rabbit antibody to neurofilament NF-M, (NB300-133), (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.
|Tested species reactivity||Bovine, Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant full length human UCHL1 purified from E. coli.|
|Contains||10mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: bovine brain whole cell homogenate, SHSY-5Y or HEK 293 cell extract, and rat spinal cord/peripheral nerve homogenate, antigen standard for UCHL1 (transient overexpression lysate).
Ubiquitin C-terminal hydrolase 1 (UCHL1) was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. The protein is extremely abundant representing 1-2% of total
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
epididymis luminal protein 117; epididymis secretory protein Li 53; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP95; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; ubiquitin thiolesterase; UCH-L1
BOS_6576; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1; UCHL1