|Tested species reactivity||Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant full length human UCHL1 purified from E. coli.|
|Storage buffer||tissue culture supernatant|
|Contains||10mM sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Protein gene product (PGP9.5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. The protein which has a molecular weight of 27kDa was first identified by high resolution two dimensional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibres at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. This antibody is of value as a marker for neurons and may be of particular use in the study of ubiquitinated cellular inclusions characteristic of several chronic human neurodegenerative diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cutaneous expression of calcium/calmodulin-dependent protein kinase II in rats with type 1 and type 2 diabetes.
480012 was used in immunohistochemistry to analyze the expression of CaMKII in the rat foot pad 2 weeks and 2 months after induction of type 1 and 2 diabetes
|Boric M,Jelicic Kadic A,Puljak L||Journal of chemical neuroanatomy (61-62:140)||2014|
Gender and gonadectomy influence on neurons in superior cervical ganglia of sexually mature rats.
480012 was used in immunohistochemistry to study the neuronal change in superior cervical ganglia due to sexual maturation and gonadal hormones
|Filipovi¿ N,Zuvan L,Ma¿ek T,Tokali¿ R,Grkovi¿ I||Neuroscience letters (563:55)||2014|
Changes in epidermal thickness and cutaneous innervation during maturation in long-term diabetes.
480012 was used in immunohistochemistry to examine the cutaneous changes in rats after the induction of diabetes.
|Jelicic Kadic A,Boric M,Vidak M,Ferhatovic L,Puljak L||Journal of tissue viability (23:7)||2014|
MicroRNA-922 promotes tau phosphorylation by downregulating ubiquitin carboxy-terminal hydrolase L1 (UCHL1) expression in the pathogenesis of Alzheimer's disease.
480012 was used in western blot to elucidate the mechanisms that regulate UCHL1 levels in Alzheimer's disease AD.
|Zhao ZB,Wu L,Xiong R,Wang LL,Zhang B,Wang C,Li H,Liang L,Chen SD||Neuroscience (275:232)||2014|
Reduced epidermal thickness, nerve degeneration and increased pain-related behavior in rats with diabetes type 1 and 2.
480012 was used in immunohistochemistry to study cutaneous changes as markers of neuropathies in two rat models of diabetes.
|Boric M,Skopljanac I,Ferhatovic L,Jelicic Kadic A,Banozic A,Puljak L||Journal of chemical neuroanatomy (53:33)||2013|
Immunofluorescent triple-staining technique to identify sensory nerve endings in human thumb ligaments.
480012 was used in immunohistochemistry (paraffin) to investigate carpometacarpal ligament innervation.
|Lee J,Ladd A,Hagert E||Cells, tissues, organs (195:456)||2012|
Conformational stabilization of ubiquitin yields potent and selective inhibitors of USP7.
480012 was used in western blot to isolate ubiquitin functional conformations that act as potent and selective USP7 inhibitors.
|Zhang Y,Zhou L,Rouge L,Phillips AH,Lam C,Liu P,Sandoval W,Helgason E,Murray JM,Wertz IE,Corn JE||Nature chemical biology (9:51)||2013|