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UFC1 Antibody (MA5-44697) in ICC/IF
Immunocytochemistry analysis of UFC1 in SH-SY5Y cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in UFC1 Monoclonal antibody (Product # MA5-44697) using a dilution of 1:400 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI.
Product Details
MA5-44697
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE65-12
Immunogen
Recombinant protein within human UFC1 aa 1-150/167.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931155
Target Information
UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (Komatsu et al., 2004 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ubiquitin-fold modifier-conjugating enzyme 1; Ufm1-conjugating enzyme 1
Gene Aliases: CGI-126; HSPC155; UFC1
UniProt ID: (Human) Q9Y3C8
Entrez Gene ID: (Human) 51506
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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