Proteintech

ULK1 Monoclonal Antibody (1D7G1)

View all (44) ULK1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (9)

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

ULK1 Antibody (68445-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ULK1 Antibody in Western Blot (WB)

ULK1 Antibody in Western Blot (WB)

ULK1 Antibody in Western Blot (WB)

ULK1 Antibody in Western Blot (WB)

ULK1 Antibody in Western Blot (WB)

Product Image Gallery

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ULK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ULK1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours.

ULK1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20,000 incubated at room temperature for 1.5 hours.

ULK1 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10,000 incubated at room temperature for 1.5 hours.

ULK1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10,000 incubated at room temperature for 1.5 hours.

ULK1 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10,000 incubated at room temperature for 1.5 hours.

Product Details

68445-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D7G1

Immunogen

ULK1 fusion protein Ag22397

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Immunogen sequence: SRKDLIGHGA FAVVFKGRHR EKHDLEVAVK CINKKNLAKS QTLLGKEIKI LKELKHENIV ALYDFQEMAN SVYLVMEYCN GGDLADYLHA MRTLSEDTIR LFLQQIAGAM RLLHSKGIIH RDLKPQNILL SNPAGRRANP NSIRVKIADF GFARYLQSNM MAATLCGSPM YMAPEVIMSQ HYDGKADLWS IGTIVYQCLT GKAPFQASSP QDLRLFYEKN KTLVPTIPRE TSAPLRQLLL ALLQRNHKDR MDFDEFFHHP FLDASPSVRK SPPVPVPSYP SSGSGSSSSS SSTSHLASPP SLGEMQQLQK TLASPADTAG FLHSSRDSGG SKDSSCDTDD FVMVPAQFPG DLVAEAPSAK PPPDSLMCSG SSLVASAGLE SHGRTPSPSP PCSSSPSPSG RAGPFSSSRC GASVPIPVPT QVQNYQRIER NLQSPTQFQT PRSSAIRRSG

Target Information

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages.

ACS infectious diseases

Jin X,Zhang C,Lin S,Gao T,Qian H,Qu L,Yao J,Du X,Feng G

Fri Aug 09 00:00:00 UTC 2024

Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages.

ACS infectious diseases

Jin X,Zhang C,Lin S,Gao T,Qian H,Qu L,Yao J,Du X,Feng G

Fri Aug 09 00:00:00 UTC 2024

Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages.

ACS infectious diseases

Jin X,Zhang C,Lin S,Gao T,Qian H,Qu L,Yao J,Du X,Feng G

Fri Aug 09 00:00:00 UTC 2024

Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages.

ACS infectious diseases

Jin X,Zhang C,Lin S,Gao T,Qian H,Qu L,Yao J,Du X,Feng G

Fri Aug 09 00:00:00 UTC 2024

Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages.

ACS infectious diseases

Jin X,Zhang C,Lin S,Gao T,Qian H,Qu L,Yao J,Du X,Feng G

Fri Aug 09 00:00:00 UTC 2024

Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages.

ACS infectious diseases

Jin X,Zhang C,Lin S,Gao T,Qian H,Qu L,Yao J,Du X,Feng G

Fri Aug 09 00:00:00 UTC 2024

Bioinformatics

Protein Aliases: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; ULK 1; UNC 51; Unc-51-like kinase 1

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Gene Aliases: ATG1; ATG1A; AU041434; hATG1; KIAA0722; mKIAA0722; ULK1; UNC51; Unc51.1

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UniProt ID: (Human) O75385, (Mouse) O70405

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Entrez Gene ID: (Human) 8408, (Rat) 360827, (Mouse) 22241

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protein serine/threonine kinase activity protein binding ATP binding Rab GTPase binding protein complex binding molecular_function protein kinase activity protein kinase binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Hsp90 protein binding non-receptor serine/threonine protein kinase

protein phosphorylation signal transduction protein localization regulation of autophagy positive regulation of autophagy macroautophagy positive regulation of macroautophagy peptidyl-serine phosphorylation neuron projection regeneration neuron projection development negative regulation of protein complex assembly cellular response to nutrient levels response to starvation protein autophosphorylation axon extension autophagosome assembly Ras protein signal transduction axonogenesis cerebellar granule cell differentiation radial glia guided migration of cerebellar granule cell receptor internalization negative regulation of collateral sprouting regulation of neurotrophin TRK receptor signaling pathway autophagy regulation of gene expression regulation of cell death phosphorylation mitophagy in response to mitochondrial depolarization

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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