Immunofluorescent analysis of UNC13 (C. elegans)-like in methanol-fixed HeLa cells using a UNC13 (C. elegans)-like polyclonal antibody (Product # PA5-22153) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 680 and 957 of UNC13B (Uniprot ID#O14795)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22153 targets UNC13 (C. elegans)-like in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-22153 immunogen is a recombinant fragment corresponding to a region within amino acids 680 and 957 of UNC13B (Uniprot ID#O14795).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.