Invitrogen

UNC84B Recombinant Rabbit Monoclonal Antibody (JG39-52)

View all (8) UNC84B antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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UNC84B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SUN2 in SK-Br-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SUN2 monoclonal antibody (Product # MA5-34800), followed by DAPI (blue).

UNC84B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of SUN2 in paraffin-embedded human prostate cancer tissue. Samples were incubated with SUN2 monoclonal antibody (Product # MA5-34800), and followed by hematoxylin.

UNC84B Antibody in Western Blot (WB) — Western blot analysis of SUN2 in rat kidney tissue lysate. Samples were incubated with SUN2 monoclonal antibody (Product # MA5-34800), at a dilution of 1:2000.

UNC84B Antibody in Flow Cytometry (Flow) — Flow cytometry of SUN2 in SK-Br-3 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with SUN2 monoclonal antibody (Product # MA5-34800) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Product Details

MA5-34800

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:100	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JG39-52
Immunogen	Recombinant protein within Human SUN2 aa 591-717 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2848708

Product Specific Information

Positive Control: Rat kidney tissue lysate, rat epididymis tissue, human tonsil tissue, human colon tissue, human prostate cancer tissue, mouse liver tissue, SK-Br-3.

Target Information

UNC84B may form a physical interaction between the nuclear envelope and the centrosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: nuclear envelope protein; Protein unc-84 homolog B; Rab5-interacting protein; Rab5IP; Sad1 unc-84 domain protein 2; Sad1/unc-84 protein-like 2; SUN domain-containing protein 2; unc-84 homolog B

Gene Aliases: FRIGG; KIAA0668; RAB5IP; SUN2; UNC84B

UniProt ID: (Human) Q9UH99

Entrez Gene ID: (Human) 25777

Molecular Function: cytoskeletal protein microtubule or microtubule-binding cytoskeletal protein non-motor microtubule binding protein

Function(s)

nuclear envelope organization mitotic spindle organization nuclear migration positive regulation of cell migration nuclear migration along microfilament centrosome localization cytoskeletal anchoring at nuclear membrane nuclear matrix anchoring at nuclear membrane

Process(es)

protein binding lamin binding microtubule binding identical protein binding

UNC84B Recombinant Rabbit Monoclonal Antibody (JG39-52)

Product Details

MA5-34800

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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