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Product Details
MA5-34781
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JG38-44
Immunogen
Recombinant protein within Human hUPF1 aa 1-120
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Wet ice
RRID
AB_2848689
Product Specific Information
Positive Control: PC-3M, A431, LOVO, human colon tissue, human prostate cancer tissue, human kidney tissue, mouse brain tissue.
Target Information
Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons. Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm and was later shown to be a component of cytoplasmic P-bodies. Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery. Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase RENT1; delta helicase; FLJ43809; FLJ46894; hUpf1; Nonsense mRNA reducing factor 1; NORF1; Regulator of nonsense transcripts 1; smg-2 homolog, nonsense mediated mRNA decay factor; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; UPF1 regulator of nonsense transcripts homolog; yeast Upf1p homolog
Gene Aliases: HUPF1; KIAA0221; NORF1; pNORF1; RENT1; smg-2; UPF1
UniProt ID: (Human) Q92900
Entrez Gene ID: (Human) 5976
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay
telomere maintenance
nuclear-transcribed mRNA catabolic process
DNA replication
DNA repair
mRNA export from nucleus
regulation of translational termination
dosage compensation by inactivation of X chromosome
telomere maintenance via semi-conservative replication
positive regulation of mRNA catabolic process
3'-UTR-mediated mRNA destabilization
histone mRNA catabolic process
cellular response to lipopolysaccharide
cellular response to interleukin-1
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