|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Bovine Complex III|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/ml per 10^6 cells|
|Western Blot (WB)||0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Use Human, Cow, Rat or Mouse heart mitochondrial lysate, HepG2 mitochondrial lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HCT116 for positive control.
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ASK1 signalling regulates brown and beige adipocyte function.
459140 was used in western blot to elucidate regulation of brown and beige adipocyte function via ASK1 signaling
|Hattori K,Naguro I,Okabe K,Funatsu T,Furutani S,Takeda K,Ichijo H||Nature communications (7:null)||2016|
The mitochondrial function of the cerebral vasculature in insulin-resistant Zucker obese rats.
459140 was used in western blot to study cerebral vasculature mitochondrial function in insulin-resistant Zucker obese rats
|Merdzo I,Rutkai I,Tokes T,Sure VN,Katakam PV,Busija DW||American journal of physiology. Heart and circulatory physiology (310:H830)||2016|
|Not Applicable||Not Cited||
Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier.
459140 was used in western blot to analyze GTPBP3 defective expression in AMPK-mediated responses
|Martínez-Zamora A,Meseguer S,Esteve JM,Villarroya M,Aguado C,Enríquez JA,Knecht E,Armengod ME||PloS one (10:null)||2015|
Dynamics of enhanced mitochondrial respiration in female compared with male rat cerebral arteries.
459140 was used in western blot to test if mitochondrial energetics of large cerebral arteries are sex dependent.
|Rutkai I,Dutta S,Katakam PV,Busija DW||American journal of physiology. Heart and circulatory physiology (309:H1490)||2015|
AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD.
459140 was used in western blot to test the requirement of AMP-activated protein kinase for exercise training-induced increases in skeletal muscle abundance of mitochondrial proteins.
|Brandauer J,Andersen MA,Kellezi H,Risis S,Frøsig C,Vienberg SG,Treebak JT||Frontiers in physiology (6:null)||2015|
Targeting FoxO1 with AS1842856 suppresses adipogenesis.
459140 was used in western blot to investigate the role of FoxO1 during adipocyte differentiation and adipogenesis
|Zou P,Liu L,Zheng L,Liu L,Stoneman RE,Cho A,Emery A,Gilbert ER,Cheng Z||Cell cycle (Georgetown, Tex.) (13:3759)||2015|
Deregulation of mitochondria-shaping proteins Opa-1 and Drp-1 in manganese-induced apoptosis.
459140 was used in western blot to investigate the role of mitochondria in manganese-induced Parkinsonism
|Alaimo A,Gorojod RM,Beauquis J,Muñoz MJ,Saravia F,Kotler ML||PloS one (9:null)||2014|
IKK¿ and alternative NF-¿B regulate PGC-1ß to promote oxidative muscle metabolism.
459140 was used in western blot to study NF-κB signaling during in lymphoid development.
|Bakkar N,Ladner K,Canan BD,Liyanarachchi S,Bal NC,Pant M,Periasamy M,Li Q,Janssen PM,Guttridge DC||The Journal of cell biology (196:497)||2012|
|Not Applicable||Not Cited||
The extrinsic and intrinsic apoptotic pathways are involved in manganese toxicity in rat astrocytoma C6 cells.
459140 was used in western blot to investigate the molecular mechanisms involved in manganese neurotoxicity
|Alaimo A,Gorojod RM,Kotler ML||Neurochemistry international (59:297)||2011|
Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses.
459140 was used in immunohistochemistry - paraffin section to investigate the effects of mitochondrial DNA damage on hippocampal neurons.
|Lauritzen KH,Cheng C,Wiksen H,Bergersen LH,Klungland A||DNA repair (10:639)||2011|