|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||a 12 amino acid peptide near the center of the mouse UROP11.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Despite its predicted molecular weight, UROP11 often migrates at a much higher apparent size in SDS-PAGE. A suggested positive control is mouse spleen tissue lysate.
PA5-20525 can be used with blocking peptide PEP-0645.
Upregulated obese product 11 (UROP11) was isolated from subtractive library for mouse hypothalamus. It is detected in numerous tissues and is upregulated in the hypothalamus and adipocytes in obese mice. UROP11 is also observed in human neural and lymphoid tissues; its expression appears to be regulated by the level of lymphoid activation. Urop11 expression is strongly upregulated both in vivo in mouse hypothalamus and in vitro in mouse neural cell lines after treatment with leptin, indicating Urop11 is a target of leptin activity and thus may play a role in obesity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.