For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
Protein Aliases: Deubiquitinating enzyme 16; Ubiquitin carboxyl-terminal hydrolase 16; ubiquitin specific protease 16; Ubiquitin thioesterase 16; ubiquitin thiolesterase 16; Ubiquitin-processing protease UBP-M; ubiquitin-specific processing protease 16; Ubiquitin-specific-processing protease 16; USP16
Gene Aliases: MSTP039; UBP-M; UBPM; USP16
UniProt ID: (Human) Q9Y5T5
Entrez Gene ID: (Human) 10600
Molecular Function: cysteine protease