OriGene

USP5 Monoclonal Antibody (OTI2H5), TrueMAB™

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USP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501289, Dilution 1:50)

USP5 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5. Positive lysates LY418652 (100 µg) and LC418652 (20 µg) can be purchased separately from OriGene.

Product Details

CF501289

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-

Product Specifications
Species Reactivity	Dog, Human, Rat
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI2H5
Immunogen	Full length human recombinant protein of human USP5 produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly, and also binds linear and Lys-63 linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Deubiquitinating enzyme 5; Isopeptidase T; testicular tissue protein Li 218; Ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5 (isopeptidase T); Ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5

Gene Aliases: ISOT; USP5

UniProt ID: (Human) P45974

Entrez Gene ID: (Dog) 486718, (Human) 8078, (Rat) 297593

Molecular Function: cysteine protease