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Invitrogen
USP9X Monoclonal Antibody (OTI2G7)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 14
USP9X Antibody (MA5-26367) in ICC/IF
Immunofluorescence analysis of Probable ubiquitin carboxyl-terminal hydrolase FAF-X was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with USP9X Monoclonal Antibody (OTI2G7) (Product # MA5-26367) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinan... View More
Product Details
MA5-26367
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI2G7
Immunogen
Human recombinant protein fragment corresponding to amino acids 2246-2570 of USP9X produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2723770
Target Information
USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9x has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity. USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme FAF-X; Drosophila fat facets related, X-linked; Fat facets homolog; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; fats facets protein related, X; hFAM; probable ubiquitin carboxyl-terminal hydrolase FAF-X; Ubiquitin carboxyl-terminal hydrolase 9X; Ubiquitin carboxyl-terminal hydrolase FAM; ubiquitin specific peptidase 9, X chromosome; ubiquitin specific protease 9 SSSRF- isoform; ubiquitin specific protease 9, X chromosome; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); Ubiquitin thioesterase FAF-X; ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X
Gene Aliases: 5730589N07Rik; AA407302; AA407699; AL022658; AL022749; DFFRX; FAF; FAF-X; Fafl; FAM; MRX99; MRXS99F; RGD1560056; USP9; USP9X
UniProt ID: (Human) Q93008, (Mouse) P70398
Entrez Gene ID: (Dog) 480885, (Human) 8239, (Mouse) 22284, (Rat) 363445
negative regulation of transcription from RNA polymerase II promoter
neuron migration
ubiquitin-dependent protein catabolic process
chromosome segregation
mitotic nuclear division
transforming growth factor beta receptor signaling pathway
female gamete generation
protein deubiquitination
BMP signaling pathway
cellular protein metabolic process
axon extension
cell division
in utero embryonic development
proteolysis
cell cycle
post-embryonic development
cerebellar cortex structural organization
hippocampus development
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
cellular response to transforming growth factor beta stimulus
neuron projection extension
cell migration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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