Invitrogen

USP9X Monoclonal Antibody (OTI2G7)

Product Image Gallery

USP9X Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a USP9X monoclonal antibody (Product # MA5-26367).

USP9X Antibody in Western Blot (WB) — Western blot analysis of USP9X in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with USP9X (Product # MA5-26367) monoclonal antibody.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Host / Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI2G7

Immunogen

Human recombinant protein fragment corresponding to amino acids 2246-2570 of USP9X produced in E.coli

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2723770

Target Information

USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9x has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity. USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Deubiquitinating enzyme FAF-X; DFFRX; Drosophila fat facets related; Drosophila fat facets related, X-linked; FAF; FAM; Fat facets homolog; Fat facets in mammals; fat facets protein related; fat facets protein related, X-linked; fat facets protein-related; Fat facets protein-related, X-linked; fats facets protein related, X; hFAM; Probable ubiquitin carboxyl-terminal hydrolase FAF-X; Ubiquitin carboxyl-terminal hydrolase FAM; ubiquitin specific peptidase 9, X chromosome; ubiquitin specific peptidase 9, X-linked; ubiquitin specific protease 9; ubiquitin specific protease 9 SSSRF- isoform; ubiquitin specific protease 9, X chromosome; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); Ubiquitin thioesterase FAF-X; ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; ubiquitin-specific protease 9; Ubiquitin-specific protease 9, X chromosome; ubiquitin-specific-process; Ubiquitin-specific-processing protease FAF-X; X chromosome; X chromosome (fat facets-like Drosophila); X-linked

Gene Aliases: 5730589N07Rik; AA407302; AA407699; AL022658; AL022749; DFFRX; FAF; FAF-X; Fafl; FAM; MRX99; MRXS99F; RGD1560056; USP9; USP9X

UniProt ID: (Human) Q93008, (Mouse) P70398

Entrez Gene ID: (Dog) 480885, (Human) 8239, (Mouse) 22284, (Rat) 363445

Function(s)