Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
Assay-Dependent | View 9 publications 9 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
GST Pull Down (GPD) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Human, Mouse |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
HWA4C4 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
RRID |
AB_1257213 |
Description: Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63). K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.
HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.
Applications Reported: This HWA4C4 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This HWA2-8°C4 antibody has been tested by immunoblot of lysine-63-linked polyubiquitin chains. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Ubiquitin is a conserved 76 amino acid polypeptide and can affect proteasomal degradation of the protein it is bound to, or mediate interactions with other proteins related to post-translational modifications. The degradation of cellular regulatory proteins by the Uubiquitin pathway is important as it controls the cellular growth and proliferation. Ubiquitin-dependent proteolysis occurs after a covalent attachment of the peptide to a lysine residue of a protein, which involves three enzymatic reactions: E1, E2 and E3. The first reaction involves ubiquitin-activating enzyme. The third reaction uses enzyme ubiquitin ligase (E3) to transfer the activated ubiquitin from E2 to a lysine residue on a protein, or directly transfers the ubiquitin from E2 to the substrate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; epididymis luminal protein 112; epididymis secretory protein Li 50; Large ribosomal subunit protein eL40; polyubiquitin B; Polyubiquitin-B; Polyubiquitin-C; Small ribosomal subunit protein eS31; Ubiquitin; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; Ubiquitin-40S ribosomal protein S27a; ubiquitin-52 amino acid fusion protein; Ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80
Gene Aliases: CEP52; CEP80; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; S27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; UBCEP80
UniProt ID: (Human) Q91888, (Human) P62987, (Human) P0CG47, (Human) P0CG48
Entrez Gene ID: (Human) 6233, (Human) 7311, (Human) 7314, (Human) 7316
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support