Western blotting analysis of ubinuclein in nuclear fraction of HeLa cells. The monoclonal antibody UBN1-01 (lane A, B) detects ubinuclein 1 (165 kDa) and an 180 kDa protein - presumable ubinuclein 2. The antibody UBN1-02 (lane C, D) detects ubinuclein 1. Antibody dilution: A, C - 1 ug/ml; B, D - 5 ug/ml.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 1-190 of ubinuclein 1.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the N-terminal portion of ubinuclein 1 (UBN1). Western blot results show a 165 kDa band.
Ubinuclein 1 (UBN1) is a ubiquitously expressed evolutionarily conserved protein which binds to proliferation-promoting genes that are repressed by formation of senescence-associated heterochromatin foci (SAHF). Ubinuclein 1 associates with various transcription factors and with histone methyltransferase activity, is indispensable for SAHF formation and appears to be a regulator of senescence. Although in most cells ubinuclein is localized to the nucleus, in cells forming tight junctions it is recruited to the cell adhesion complexes, dependently on the cell density.
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